Molecular characterization and distribution of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi from yaks in Tibet, China

Table 3 Primer sequences and reaction conditions used in nested PCR amplifications

Locus	Primer sequences (5′-3′)	Nucleotide fragment (bp)	Annealing temperature (°C)	Reference
Cryptosporidium SSU rRNA	SSU-F2: TTCTAGAGCTAATACATGCG	840	55	[57]
	SSU-R2:CCCATTTCCTTCGAAACAGGA		55
	SSU-F3:GGAAGGGTTGTATTTATTAGATAAAG		55
	SSU-R4:CTCATAAGG TGCTGAAGGAGTA		55
G. duodenali SSU rRNA	Gia2029: AAGTGTGGTGCAGACGGACTC	292	55	[58]
	Gia2150c: CTGCTGCCGTCCTTGGATGT		55
	RH11: CATCCGGTCGATCCTGCC		59
	RH4: AGTCGAACCCTGATTCTCCGCCCAGG		59
G. duodenalis tpi	AL3543: AAATIATGCCTGCTCGTCG	530	50	[59]
	AL3546: CAAACCTTITCCGCAAACC		50
	AL3544: CCCTTCATCGGIGGTAACTT		50
	AL3545: GTGGCCACCACICCCGTGCC		50
E. bieneusi ITS	EBITS3: GGTCATAGGGATGAAGAG	389	57	[60]
	EBITS4: TTCGAGTTCTTTCGCGCTC		57
	EBITS1: GCTCTGAATATCTATGGCT		55
	EBITS2.4: ATCGCCGACGGATCCAAGTG		55

ISSN: 1746-6148