Table 2 Specific antibodies used for Western immunoblotting and Immmunohistochemistry
From: Effects of prostaglandin F2α (PGF2α) on cell-death pathways in the bovine corpus luteum (CL)
Antibodies | Clone | Biological source | Commercial source | Dilution for WB | Dilution for IHC |
|---|---|---|---|---|---|
Anti-ACTB | monoclonal | mouse | Sigma, #A2228 | 1:10.000 | |
Anti-CASP3 | polyclonal | rabbit | Abcam, ab44976 | 1:500 | |
Anti-CASP8 | monoclonal | rabbit | Abcam, ab108333 | 1:1.000 | |
Anti-BAX | polyclonal | rabbit | Abcam, ab53154 | 1:500 | |
Anti-BCL2 | polyclonal | rabbit | Abcam, ab59348 | 1:500 | |
Anti-RIPK1 | polyclonal | rabbit | Sigma, #SAB3500420 | 1:1.000 | 1:200 |
Anti-RIPK3 | polyclonal | rabbit | Sigma, #SAB2102009 | 1:1.000 | 1:100 |
Anti-CYLD | polyclonal | rabbit | Abcam, 137,524 | 1:500 | 1:100 |
Anti-MLKL | monoclonal | rabbit | Abcam, ab187091 | 1:1.000 | 1:100 |