Table 1 Sequences for primers and accession numbers for genes
From: Effects of prostaglandin F2α (PGF2α) on cell-death pathways in the bovine corpus luteum (CL)
Gene name | Sequence of nucleotide | GenBank Accession No. | PCR products size |
|---|---|---|---|
CASP3 | 5′ TGGTGCTGAGGATGACATGG ‘3 5′ GAGCCTGTGAGCGTGCTTTT’3 | NM_001077840.1 | 163 bp |
CASP8 | 5′ CTGAGAGAAGAGGCCCGTGA ‘3 5′ CCCGGCTTAGGAACTTGAGG ‘3 | DQ319070.1 | 173 bp |
BAX | 5′ GTGCCCGAGTTGATCAGGAC ‘3 5′ CCATGTGGGTGTCCCAAAGT ‘3 | U92569.1 | 126 bp |
BCL2 | 5′ GAGTTCGGAGGGGTCATGTG ‘3 5′ GCCTTCAGAGACAGCCAGGA ‘3 | U92434.1 | 203 bp |
RIPK1 | 5′ GCAATAGCTCCAAGCAGGTC ‘3 5′ TGTGCAGCAGGAAGTCATTC ‘3 | NM_001035012 | 148 bp |
RIPK3 | 5′ CCAGAGAGAGCAGGTTCCAC ‘3 5′ AATCAGGCGGTTGTTGTTTC ‘3 | NM_001101884.2 | 219 bp |
CYLD | 5′ GCAATGCTCAAGTCCACCT ‘3 5′ CGTGTCTCCAGTCCCAGTC ‘3 | XM_015475764.2 | 96 bp |
MLKL | 5′ ACTTCCATCAGCCGACAAAC ‘3 5′ CTCCCAGAGGACAATTCCAA ‘3 | XM_024978879.1 | 144 bp |
GAPDH | 5′ CACCCTCAAGATTGTCAGCA ‘3 5′ GGTCATAAGTCCCTCCACGA ‘3 | BC102589 | 103 bp |
ACTB | 5′ GAGGATCTTCATGAGGTAGTCTGTCAGG ‘3 5′ CAACTGGGACGACATGGAGAAGATCTGGCA ‘3 | AY141970 | 349 bp |
RN18S1 | 5′ AAGTCTTTGGGTTCCGGG ‘3 5’GGACATCTAAGGGCATCACA’3 | AF176811 | 365 bp |