Table 4 Oligonucleotide primer sequences for amplification of target equine genes
Gene | Oligo | Sequence (5′-3′) | PCR Product size (bp) | Annealing temperature (°C) | Sequence accession number(s) | Reference(s) |
|---|---|---|---|---|---|---|
GAPDH | Forward | GGTGAAGGTCGGAGTAAACG | 106 | 64 | AF157626; AF083897 | Beekman, 2011 |
Reverse | AATGAAGGGGTCATTGATGG | |||||
HRPT | Forward | AATTATGGACAGGACTGAACGG | 121 | 62 | AY372182 | Beekman, 2011 |
Reverse | ATAATCCAGCAGGTCAGCAAAG | |||||
SDHA | Forward | GAGGAATGGTCTGGAATACTG | 91 | 62 | DQ402987 | Beekman, 2011 |
Reverse | GCCTCTGCTCCATAAATCG | |||||
RPL-32 | Forward | GGGAGCAATAAGAAAACGAAGC | 138 | 62 | CX594263 | Beekman, 2011 |
Reverse | CTTGGAGGAGACATTGTGAGC | |||||
IL-1β | Forward | ACCATAAATCCCTGGTGCTG | 179 | 64 | D42147; U92481; D42165 | Beekman, 2012 |
Reverse | CGTCCCACAAGACAGGTACA | |||||
IL-4 | Forward | TCGTGCATGGAGCTGACTGTA | 151 | 64 | L06010; AF035404 | Beekman, 2012 |
Reverse | GCCCTGCAGATTTCCTTTCC | |||||
IL-5 | Forward | AAACTGTCCAAGGGGATGCT | 169 | 64 | U91947 | Beekman, 2012 |
Reverse | TCCGTTGTCCACTCAGTGTT | |||||
IL-6 | Forward | AGCAAGGAGGTACTGGCAGA | 173 | 62 | U64794; AF005227; AF041975 | Beekman, 2012 |
Reverse | CCTTTTCACCCTTGAACTCG | |||||
IL-8 | Forward | CGCACTCCAAACCTTTCAAT | 165 | 62 | AY184956; AF062377 | Beekman, 2012 |
Reverse | TCAAAAACGCCTGCACAATA | |||||
IL-10 | Forward | ATCGATTTCTGCCCTGTGAA | 174 | 62 | U38200 | Beekman, 2012 |
Reverse | CGTTCCCTAGGATGCTTCAG | |||||
IL-12 p35 | Forward | CATGAATGCCAAGCTGTTGA | 185 | 64 | Y11130 | Beekman, 2012 |
Reverse | AGGCATGAAGAAGGATGCAG | |||||
IL-17 | Forward | TATCGTGAAGGCGGGAATAG | 210 | 62 | EU744563 | Beekman, 2012 |
Reverse | TCCCAGATCACAGAGGGGTA | |||||
IL-23 | Forward | CTGGCCTGGAGTGCACATC | 296 | 62 | AY704416 | Hughes, 2011 |
Reverse | TTGTAGTCTCAGCATCTCCCTCTTC | |||||
IFN-γ | Forward | CTATTACTGCCAGGCCGCGTT | 404 | 64 | U04050; D28520 | Giguère, 1999 |
Reverse | TCCTCTTCCGCTTCCTCAGGTT | |||||
Eotaxin-2 | Forward | CCTGAGAGCCGAGTGGTAAG | 152 | 64 | ENSECAT00000023737 | Beekman, 2012 |
Reverse | TTCTTGGCAGCCAGATTCTT | |||||
TNF-α | Forward | CTTGTGCCTCAGCCTCTTCTCCTTC | 1385 | 64 | M64087 | Giguère, 1999 |
Reverse | CAGCTGGTTGTCTGTCAGCTTC |