Table 2 Relative gene expression in 3 healthy horses before and after treatment with 20 mg dexamethasone IM SID for 10 days. The normalization factor (calculated from multiple reference genes) has a value of 1, and genes of interest are either up-regulated or down-regulated in response to treatment. * indicates a significant difference (p < 0.05)
Gene | Expression | Regulation | Standard Error | 95% C.I. | P value |
|---|---|---|---|---|---|
IL-1β | 2.33 | DOWN | 0.01–4.28 | 0.01–21.33 | 0.35 |
IL-4 | 1.56 | DOWN | 0.16–4.04 | 0.05–7.53 | 0.4 |
IL-5 | 2.56 | DOWN | 0.01–17.84 | 0.01–29.49 | 0.5 |
IL-6 | 1.35 | UP | 0.03–71.96 | 0.02–84.78 | 0.59 |
IL-8 | 1.89 | DOWN | 0.06–3.14 | 0.02–5.05 | 0.29 |
IL-10 | 2.63 | DOWN | 0.06–1.80 | 0.03–2.72 | 0.11 |
IL-12 | 2.00 | DOWN | 0.09–1.98 | 0.06–5.09 | 0.19 |
IL-17 | 1.75 | DOWN | 0.02–11.83 | 0.01–38.45 | 0.53 |
IL-23 | 3.70 | DOWN | 0.02–2.85 | 0.01–4.56 | 0.11 |
Eotaxin-2 | 1.75 | DOWN | 0.09–6.38 | 0.02–19.53 | 0.42 |
TNF-α | 3.03 | DOWN | 0.07–1.12 | 0.05–3.44 | 0.023* |