Fig. 1

Serum specific IgG response of experimental lambs subjected to 1000 L3/ dose trickle Haemonchus infection against rHc23 along the experiment. Group 1 (●), Group 2 (■) and Group 3 (○). Data given (Optical density, OD) are means ± standard error of the mean (SEM) of the individual OD values from each animal and time point (Group 1: 6 lambs; Group 2: 7 lambs; Group 3: 4 lambs). Insert: Immune recognition of ASE of H.contortus. Lanes 1–7: individual sera of vaccinated lambs (Group 1); BI: pooled sera from adjuvant control group (Group 2); (−): pooled sera of lambs from untreated + infected group (Group 3). Arrows: days of vaccination. Vertical bars: days of infection. MW: molecular weight markers. *: Membrane stained with Amido black