Fig. 3

Dot blot assay prepared using antibodies raised against E. coli-expressed BEFV N protein. a Recombinant N protein (68 kDa) expression was induced for 0, 2, 4, and 6 h in E. coli and analyzed by SDS-PAGE (upper panel). Rabbit anti-BEFV antiserum was employed to identify rN protein (lower panel). b Protein samples of BHK-21 cell culture supernatant (BHK-21 sup.), purified BEFV (BEFV), and rN protein (rN) were separated by SDS-PAGE (upper panel). In the lower panel, Western blotting was then performed and the samples were co-probed by two different antibodies: rabbit anti-BEFV antiserum (red color), and mouse anti-rN antiserum (blue color). c Dot blot assays were set up with raised rabbit anti-rN antiserum as the primary antibody. Two-fold serial dilutions of denatured (upper panel) or native (lower panel) BEFV test samples, or BHK-21 cell supernatant, were applied for BEFV detection