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Table 2 Fermentation parameters of the dietary substrates after 24 h of in vitro incubation

From: Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep

Parameter Rumen fluid inoculum Fresh fecal inoculum
  Mix1 Mix2 MH Concentrate Mix1 Mix2 MH Concentrate
pH 6.86 7.04 7.16 6.92 7.28 7.32 7.41 7.13
NH3-N (mg/L) 130 147 122 126 158 218 167 127
Total gas (mL/g DM) 180 178 120 222 91 90 61 140
CH4 (mmol) 6.99 6.68 7.90 7.62 6.49 6.21 7.05 7.06
Total SCFA (mmol/L) 48.3 47.8 42.4 53.4 46.8 47.2 43.1 49.3
Acetate (mol%) 60.0 60.3 69.1 63.4 66.3 65.9 66.1 67.2
Propionate (mol%) 16.4 16.4 17.2 19.1 20.1 20.9 20.2 23.7
Butyrate (mol%) 10.9 10.3 10.4 13.1 3.14 2.82 2.50 8.82
Total ciliate protozoa (103/mL) 94 99 114 160
Entodinium spp. (103/mL) 91 96 111 155
Epidinium spp. (103/mL) 2.96 3.19 3.17 5.39
  1. Mix1 herbal mixture 1, Mix2 herbal mixture 2, Concentrate commercial concentrate composed of barley, soybean meal, wheat bran, bicarbonate and mineral–vitamin premix, MH meadow hay