Fig. 3
From: Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain
Purification and specificity analysis of the anti-MmPrdx6 monoclonal antibody. a mAbs against MmPrdx6 analysed via SDS-PAGE. M: Protein marker (Sangon, China); Lane 1: Ascites fluid harvested from BALB/c mice inoculated with the B5F2 hybridoma cell line secreting anti-MmPrdx6 mAbs; Lane 2: Purified mAbs against MmPrdx6. b Specificity of anti-MmPrdx6 mAbs determined through western blotting. M: Protein marker (Sangon, China); Lane 1: Purified rMmPrdx6 protein; Lane 2: Total protein from uninduced E. coli BL21(DE3) cells harbouring pET-28a-Prdx6; Lane 3: Total protein from the RAW264.7 murine macrophage cell line