Table 2 Primer sequences and PCR conditions used for the detection of tick-borne pathogens in this study
From: Evaluation of the efficacy of ivermectin against Theileria orientalis infection in grazing cattle
Tick-borne pathogen | Specific genes | Sequence (5′ to 3′) | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
Anaplasma spp. | 16S rRNA | TACCTCTGTGTTGTAGCTAACGC CTTGCGACATTGCAACCTATTGT | 58 | 429 | [26] |
Babesia spp. | 18S rRNA | GTTTCTGMCCCATCAGCTTGAC CAAGACAAAAGTCTGCTTGAAAC | 61 | 420–440 | [27] |
Ehrlichia spp. | 16S rRNA | CGGAATTCCTAGTGTAGAGG AGGAGGGATACGACCTTCAT | 58 | 340 | [26] |
Rickettsia spp. | 16S rRNA | TAGGGGATGATGGAATTCCTA CCCCCGTCA ATTCCTTTGAG | 58 | 252 | [26] |
Theileria orientalis | MPSP* | CACGCTATGTTGTCCAAGAG TGTGAGACTCAATGCGCCTA | 55 | 830 | [28] |