The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six months

Table 2 Protein concentrations in fresh activated platelet-rich plasma (PRP0) and liquid nitrogen frozen-thawed activated PRP (LN0)

	PRP0	LN0	LN0/PRP0	p-value
IGF-1	340 ± 21.5 ng/ml	333 ± 24.3 ng/ml	0.98	0.3141
PDGF-BB	6058 ± 696 pg/ml	4942 ± 397 pg/ml	0.85	0.1211
TGF-β1	4671 ± 896 pg/ml	4329 ± 911 pg/ml	0.92 †	0.0162
MMP-9	11,156 ± 2743 pg/ml	21,762 ± 3457 pg/ml	2.19 †	0.0021

Values reported as mean (n = 6) ± standard error. (†) indicates significant difference between PRP0 and LN0 using a paired t-test, p < 0.05. Insulin-like growth factor (IGF-1), platelet-derived growth factor-BB (PDGF-BB), transforming growth factor-β1 (TGF-β1), matrix metalloproteinase-9 (MMP-9), fold-change of liquid nitrogen frozen-thawed activated PRP at time 0 to fresh activated PRP at time 0 (LN0/PRP0)

ISSN: 1746-6148