Fig. 2

Determination of the limit of detection (X-axis for time–minutes, Y-axis for OD 650 nm). 1, the original solution with 7.5 × 10⁷ copies/μL; 2–8, dilutions of 10¹⁻ 10⁷ with 7.5 × 10⁶–7.5 copies/μL. Positive amplification products were detected from Nos. 1–7. The quantification cycle (Cq) values for the real-time PCR of the templates with the same dilutions as above in LAMP (No:1–7) were 12.7, 15.8, 19.1, 22.2, 25.6, 28.8 and 31.8, respectively