Skip to main content

Table 3 Sensitivity of the conventional PCR, oligonucleotide microarray, and real-time PCR

From: Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases

No EID50 Conventional PCR Oligonucleotide microarray Real-time PCR
H5 H7 H9 H5 H7 H9 IBV NDV H5 H7 H9 H5 H7 H9 IBV NDV H5 H7 H9 H5 H7 H9 IBV NDV
M M M
1 1 × 105 +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++
2 1 × 104 +++ +++ +++ +++ +++ +++ +++ ++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++
3 1 × 103 ++ ++ ++ ++ ++ ++ ++ + +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++
4 1 × 102 + + + + + + + ̶ +++ +++ +++ +++ +++ +++ ++ +++ ++ ++ ++ ++ +++ ++ ++ +++
5 1 × 101 ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ +++ +++ +++ +++ +++ +++ ++ +++ + + + ++ ++ + + ++
6 1 × 100 ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ++ ++ ++ ++ ++ ++ + ++ + + + + + ̶ ̶ +
7 1 × 10−1 ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ + + + + + + ̶ + ̶ ̶ ̶ + ̶ ̶ ̶ +
8 1 × 10−2 ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶ ̶
  1. Serial tenfold dilutions of virus from 105 to 10−2 EID50 were tested for the conventional PCR, oligonucleotide microarray, and real-time PCR. The products of conventional PCR were separated using 1% agarose gel electrophoresis. +++ represents a very bright band/spot/signal, ++ represents a moderately bright band/spot/signal, + represents a weak band/spot/signal, and − represents no band/spot/signal