Table 2 Primers and probes
Name | Sequence(5′-3′) | Targeted gene and virus type | Length |
|---|---|---|---|
H9 probe | TTCGACTGTCGCCTCATCTCTTG | Haemagglutinin gene of AIV subtype H9 | 157 |
H9-F | Biotin-CAGAACAAGAAGGCAGCAA | ||
H9-R | AATGTGATGACCARTGCATGG | ||
H7 probe | GGTTTAGCTTCGGGGCATCATG | Haemagglutinin gene of AIV subtype H7 | 105 |
H7-F | Biotin-CCATTRCAATGGCTAGAAG | ||
H7-R | AATAGAATACAGATWGACCCAGT | ||
H5 probe | GCCTCAAACTGAGTGTTCATTTTGT | Haemagglutinin gene of AIV subtype H5 | 210 |
H5-F | Biotin-GTACCACCATAGCAATGAGCAG | ||
H5-R | AGTCCAGACATCTAGGAATCCGT | ||
M probe | TCGGCTTTGAGGGGGCCTGA | M gene of all subtypes of AIV | 163 |
M-F | Biotin-ATGAGYCTTCTRACCGAGGTCG | ||
M-R | GAGGTGACAGGATTGGTCTTGTC | ||
IBV probe | CGCCCATCCTTAATACCTTCCTCA | N gene of IBV and all pathotypes of IBV | 175 |
IBV-F | Biotin-GTARGGAGGGNAATTTTGGTGATGA | ||
IBV-R | ACACACTSRTCACAAATYTTYACATAATTA | ||
NDV probe | GAGGTGTCAAGYTCTTCTATCACAGAACC | F gene of NDV and all pathotypes of NDV | 107 |
NDV-F | Biotin-GTCCCRAARGTRGTGACACA | ||
NDV-R | GGGAAYTGTCACTATYCTDGTACA |