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Table 5 Sequences and melting temperature (Tm) of the oligos for EMSA

From: A single nucleotide variant in the promoter region of the CCR5 gene increases susceptibility to arthritis encephalitis virus in goats

Oligo name

Sequence 5′-3’

Tm

CCR5_SNPg.1059_C_f

CTGGCTTCCACAAACACCAAGTGGGTCAGA

76 °C

CCR5_SNPg.1059_C_r

TCTGACCCACTTGGTGTTTGTGGAAGCCAG

CCR5_SNPg.1059_T_f

CTGGCTTCCACAAATACCAAGTGGGTCAGA

73.3 °C

CCR5_SNPg.1059_T_r

TCTGACCCACTTGGTATTTGTGGAAGCCAG

  1. The polymorphic nucleotides of the SNPg.1059 are given in bold
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BMC Veterinary Research

ISSN: 1746-6148

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