Table 5 Primers used to detect ESBL-resistance genes and genotypes in broiler farm isolates
Target gene | Primer | Sequence (5 → 3) | Annealing Temp (°C) | Size (bp) | Ref |
|---|---|---|---|---|---|
bla CTX-M | CTX-M-F CTX-M-R | ATGTGCAGYACCAGTAARGTKATGGC TGGGTRAARTARGTSACCAGAAYSAGCGG | 55 | 592 | [29] |
bla CTX-M-1group | CTX-M-1-F CTX-M-1-R | GGTTAAAAAATCACTGCGTC TTACAAACCGTYGGTGACGA | 50 | 873 | [29] |
bla CTX-M-15 | CTX-M-15-F CTX-M-15-R | CACACGTGGAATTTAGGGACT GCCGTCTAAGGCGATAAACA | 50 | 995 | [30] |
bla CTX-M-2group | CTX-M-2-F CTX-M-2-R | ATGATGACTCAGAGCATTCGCCGC TCAGAAACCGTGGGTTACGATTTT | 56 | 876 | [31] |
bla CTX-M-8group | CTX-M-8-F CTX-M-8-R | TGATGAGACATCGCGTTAAG TAACCGTCGGTGACGATTTT | 52 | 666 | [32] |
bla CTX-M-9group | CTX-M-9-F CTX-M-9-R | GTGACAAAGAGAGTGCAACGG ATGATTCTCGCCGCTGAAGCC | 55 | 856 | [33] |
bla CTX-M-25group | CTX-M-25-F CTX-M-25-R | GCACGATGACATTCGGG AACCCACGATGTGGGTAGC | 52 | 327 | [34] |
bla TEM | TEM-F TEM-R | TTGGGTGCACGAGTGGGTTA TAATTGTTGCCGGGAAGCTA | 55 | 506 | [35] |
bla SHV | SHV-F SHV-R | TCGGGCCGCGTAGGCATGAT AGCAGGGCGACAATCCCGCG | 52 | 628 | [35] |