Fig. 5
Determination of neutrophil extracellular traps (NETs) and serpin B1 in the synovial fluid of heifers with acute ruminal acidosis (ARA). a cf-DNA measurement at 0, 9, and 24 h. Relative fluorescence units (RFU). b Immunofluorescence of synovial fluid at 0 h and 24 h after the induction of ARA. Histone H3cit and DNA at 24 h post induction was observed. A representative western blot of synovial fluid to detect H3cit at 24 h post induction is shown. Scale bar 100 μm and 10 μm for 0 h and 24 h respectively. c Immunoblot of serpin B1 at 0, 9, and 24 h in the synovial fluid. A bar graph of the relative densitometry units (RDU) of serpin B1 is shown. Each bar shows the arithmetic mean ± SEM. N.D. none detected