Protective effect of chicken egg yolk immunoglobulins (IgY) against enterotoxigenic Escherichia coli K88 adhesion in weaned piglets

Table 2 Cytokines primers used for the real time PCR

Target gene	Orientation	Sequence (5′-3′)	T m (°C)	Product size (bp)
β-actin	Forward	AGTTGAAGGTGGTCTCGTGG	57.4	216
β-actin	Reverse	TGCGGGACATCAAGGAGAAG
TNF-α	Forward	ACAGGCCAGCTCCCTCTTAT	53.9	102
TNF-α	Reverse	CCTCGCCCTCCTGAATAAAT
IL-22	Forward	AGCAAGCGTGAAGGTGCGGTT	60	169
IL-22	Reverse	GCGGACATCTGGGAGCCCTTT
IL-6	Forward	GGCAAAAGGGAAAGAATCCAG	57	87
IL-6	Reverse	CGTTCTGTGACTGCAGCTTATCC
IL-1β	Forward	CCTGGACCTTGGTTCTCT	53	123
IL-1β	Reverse	GGATTCTTCATCGGCTTCT

Abbreviations: TNF-α tumor necrosis factor alpha, IL-22 interleukin 22, IL-6 interleukin 6, IL-1β interleukin 1β, T m (°C) melting temperature

ISSN: 1746-6148