Table 2 Equine primer and probe sequences used for gene expression analyses

18S, 18 small ribonucleic acid

Forward, 5′- GCCGCTAGAGGTGAAATTCT-3′

Reverse, 5′- TCGGAACTACGACGGTATCT −3′

Probe, 5′- AAGACGGACCAGAGCGAAAGCAT-3′

ACAN, aggrecan

Forward, 5′-GAGGAGATGGAGGGTGAGGT −3′

Reverse, 5′-GATGGTGATGTCCTCCTCGC-3′

Probe, 5′-TTCACCTGTGTAGCAGATGGCGTC-3’

COL2b, type II collagen

Forward, 5’-GCTACACTCAAGTCCCTCAAC-3′

Reverse, 5′-ATCCAGTAGTCTCCGCTCTT-3′

Probe, 5′-ACCTGAAACTCTGCCACCCTGAAT-3’

SOX9, SRY-box 9

Forward, 5’-CTGGAGACTGCTGAACGAGA-3′

Reverse, 5′-GAGATGTGTGTCTGCTCCGT − 3′

Probe, 5′-AGAAGGACCACCCGGACTACAAGTA-3’