Fig. 9

Immunolocalization of PGFS and PTGFR in bovine early- and late-cleaved embryos at early different stages of development. The intracellular localization of PGFS (A, B, D, E, G, H), and PTGFR (a, b, d, e, g, h) was detected by green fluorescence in the early- and late-cleaved groups of embryos, where A, D, G and a, d, g images represent the early-cleaved embryos, but the B, E, H and b, e, h images represent the late-cleaved groups of embryos. The nuclei of the cells are depicted by blue fluorescent dots. Panels C, F, I and c, f, I present images of the negative controls for PGFS and PTGFR, respectively. A-C - images of PGFS staining in the early stages of development. a-c – images of PTGFR staining in the early stages of development. D-F - images of PGFS staining in the morula stage of embryo development. d-f - images of PTGFR staining in the morula stage of embryo development. G-I - images of PGFS staining in the blastocyst stage of embryo development. g-i - images of PTGFR staining in the blastocyst stage of embryo development