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Table 5 Relative mRNA levels of MyoD, Myogenin, IFG-1, GH, PAX7 and PCNA in the muscle tissues at days 1, 3, 5, 7 post hatch in broiler chicks subjected to different thermal manipulation protocols during embryonic life (ED12–18)

From: Thermal manipulation of the broilers embryos: expression of muscle markers genes and weights of body and internal organs during embryonic and post-hatch days

Post-Hatch days

Parameters

Groups

Control

TM1

TM2

TM3

TM4

Day 1

MyoD

1 ± 0.01a

1.80 ± 0.03b

1.13 ± 0.02a

3.46 ± 0.06c

1.25 ± 0.02d

Myogenin

1 ± 0.01a

0.89 ± 0.02a

0.25 ± 0.02b

0.71 ± 0.02c

0.69 ± 0.02c

IGF-1

1 ± 0.01a

0.63 ± 0.02b

0.44 ± 0.03b

0.39 ± 0.01b

0.62 ± 0.03b

GH

1 ± 0.02a

1.26 ± 0.01b

1.15 ± 0.01c

1.15 ± 0.04c

1.09 ± 0.01c

PAX7

1 ± 0.01a

1.82 ± 0.01b

1.40 ± 0.06c

1.91 ± 0.07b

1.21 ± 0.07c

PCNA

1 ± 0.01a

0.65 ± 0.02b

0.55 ± 0.02b

0.40 ± 0.02b

0.31 ± 0.02b

Day 3

MyoD

1 ± 0.01a

2.46 ± 0.03b

0.82 ± 0.02a

0.65 ± 0.02a

1.22 ± 0.02a

Myogenin

1 ± 0.01a

2.05 ± 0.03b

1.28 ± 0.03a

0.59 ± 0.02a

1.46 ± 0.01a

IGF-1

1 ± 0.01a

2.47 ± 0.06b

0.63 ± 0.03d

0.65 ± 0.03d

1.56 ± 0.03c

GH

1 ± 0.02a

1.73 ± 0.06b

1.01 ± 0.01a

0.04 ± 0.04c

1.55 ± 0.01b

PAX7

1 ± 0.02a

3.29 ± 0.04b

1.55 ± 0.01c

1.46 ± 0.02c

1.99 ± 0.02c

PCNA

1 ± 0.03a

3.18 ± 0.09b

1.05 ± 0.01a

1.03 ± 0.02a

1.63 ± 0.05c

Day 5

MyoD

1 ± 0.02a

0.95 ± 0.03a

0.79 ± 0.03a

0.35 ± 0.02b

0.13 ± 0.01b

Myogenin

1 ± 0.06a

1.45 ± 0.02a

3.49 ± 0.23b

4.36 ± 0.19c

10.54 ± 0.27d

IGF-1

1 ± 0.02 a

1.33 ± 0.05a

0.64 ± 0.03a

0.34 ± 0.01b

0.15 ± 0.01b

GH

1 ± 0.03a

1.37 ± 0.06a

6.77 ± 0.02b

7.27 ± 0.02c

8.50 ± 0.07d

PAX7

1 ± 0.03a

1.11 ± 0.04b

1.51 ± 0.02b

1.17 ± 0.01b

1.15 ± 0.03b

PCNA

1 ± 0.09a

1.33 ± 0.04a

1.31 ± 0.02a

1.69 ± 0.06b

5.01 ± 0.13c

Day 7

MyoD

1 ± 0.04a

1.46 ± 0.11a

1.10 ± 0.03a

1.12 ± 0.04a

2.09 ± 0.06b

Myogenin

1 ± 0.01a

2.04 ± 0.22b

1.28 ± 0.05a

0.05 ± 0.19d

4.63 ± 0.19c

IGF-1

1 ± 0.07a

1.54 ± 0.11b

1.70 ± 0.05b

1.65 ± 0.03b

0.14 ± 0.02c

GH

1 ± 0.01a

2.44 ± 0.15b

1.37 ± 0.07a

1.02 ± 0.20a

5.80 ± 0.10d

PAX7

1 ± 0.03

1.01 ± 0.06

1.18 ± 0.05

1.01 ± 0.01

1.01 ± 0.03

PCNA

1 ± 0.03a

1.65 ± 0.06b

1.28 ± 0.05b

1.01 ± 0.06a

2.56 ± 0.20c

  1. TM: Thermal manipulation; MyoD: Myogenic differentiation gene; IGF-1: Insulin-like growth factor 1; GH: Growth hormone; Pax7: Paired box transcription factor; PCNA: Proliferating cell nuclear antigen. a-d Within the same row, means ± SD with different superscripts differ significantly (P < 0.05)