Table 2 Virulence factors, sequence of primers, references and expected amplicon size of target gene used in this study
Gene | Virulence factor | Primer sequence (5′-3′) | Reference | Amplicon size (bp) |
|---|---|---|---|---|
chiA | Chitinase | F: GGAAGATGGCGTGATTGACT R: GGCATCAATTTCCCAAGAGA | [21] | 232 |
vhpA | Metalloprotease | F: CTGAACGACGCCCATTATTT R:CGCTGACACATCAAGGCTAA | [21] | 201 |
luxR | Quorum sensing factors | F: ATGGACTCAATTGCAAAGAG R: TTAGTGATGTTCACGGTTGT | [21] | 618 |
flaC | Flagella of V. anguillarum | F: AAATCATTCCAAATCGGTGC R: TCTTTGATTCGGCTCTTA | [25] | 580 |
hlyA | Haemolysin of V. cholera | F: GGCAAACAGCGAAACAAATAC C R: CTCAGCGGGCTAATACGGTTTA | [48] | 738 |
toxR Vc | Toxin of V. cholera | F: ATG TTC GGA TTA GGA CAC R: TAC TCA CAC ACT TTG ATG GC | [49] | 883 |
tlh | Thermolabile haemolysin of V. parahaemolyticus | F: AAAGCGGATTATGCAGAAGCACTG R: GCTACTTTCTAGCATTTTCTCTGC | [27] | 450 |
tdh | Thermostable direct haemolysin (TDH) of V. parahaemolyticus | F: GTAAAGGTCTCTGACTTTTGGAC R: TGGAATAGAACCTTCATCTTCACC | [27] | 269 |
trh | TDH-related haemolysin (TRH) of V. parahaemolyticus | F: TTGGCTTCGATATTTTCAGTATCT R: CATAACAAACATATGCCCATTTCCG | [27] | 500 |