Identification of Escherichia coli from broiler chickens in Jordan, their antimicrobial resistance, gene characterization and the associated risk factors

Table 8 PCR target genes, primer sequence, PCR product size and annealing temperature

Target gene	Primer sequence	PCR product size (bp)	Annealing Temp.
tetA	tetAF GCT ACA TCC TGC TTG CCT TC	210	55
tetracycline	tetAR CAT AGA TCG CCG TGA AGA GG	210	55
tetB	tetBF TTG GTT AGG GGC AAG TT1 T TG	659	55
tetracycline	tetBR GTA ATG GGC CAA TAA CAC CG	659	55
blaTEM	TEMF ATT CTT GAA GAC GAA AGG GC	1150	60
beta lactams	TEMR A CG CTC AGT GGA ACG AAA AC	1150	60
blaSHV	SHVF CAC TCA AGG ATG TAT TGT G	885	60
beta lactams	SHVR TTA GCG TTG CCA GTG CTC G	885	60
sul1 sulphonamide	sul1F CTT CGA TGA GAG CCG GCG GC	417	68
sul1 sulphonamide	sul1R GCA AGG CGG AAA CCC GCG CC	417	68
sul2 sulphonamide	sul2F AGG GGG CAG ATG TGA TCG AC	249	58
sul2 sulphonamide	sul2R GCA GAT TTC GCC AAT TG	249	58
Cat1 chloramphenicol	catF CCT GCC ACT CAT CGC AGT	623	55
Cat1 chloramphenicol	catR CCA CCG TTG ATA TAT CCC	623	55
int1 integrons	int1F CCT CCC GCA CGA TGA TC	280	55
int1 integrons	int1R TCC ACG CAT CGT CAG GC	280	55

ISSN: 1746-6148