Skip to main content

Table 8 PCR target genes, primer sequence, PCR product size and annealing temperature

From: Identification of Escherichia coli from broiler chickens in Jordan, their antimicrobial resistance, gene characterization and the associated risk factors

Target gene Primer sequence PCR product
size (bp)
Annealing
Temp.
tetA tetAF GCT ACA TCC TGC TTG CCT TC 210 55
tetracycline tetAR CAT AGA TCG CCG TGA AGA GG
tetB tetBF TTG GTT AGG GGC AAG TT1 T TG 659 55
tetracycline tetBR GTA ATG GGC CAA TAA CAC CG
blaTEM TEMF ATT CTT GAA GAC GAA AGG GC 1150 60
beta lactams TEMR A CG CTC AGT GGA ACG AAA AC
blaSHV SHVF CAC TCA AGG ATG TAT TGT G 885 60
beta lactams SHVR TTA GCG TTG CCA GTG CTC G
sul1 sulphonamide sul1F CTT CGA TGA GAG CCG GCG GC 417 68
sul1R GCA AGG CGG AAA CCC GCG CC
sul2 sulphonamide sul2F AGG GGG CAG ATG TGA TCG AC 249 58
sul2R GCA GAT TTC GCC AAT TG
Cat1 chloramphenicol catF CCT GCC ACT CAT CGC AGT 623 55
catR CCA CCG TTG ATA TAT CCC
int1 integrons int1F CCT CCC GCA CGA TGA TC 280 55
int1R TCC ACG CAT CGT CAG GC