Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis

Table 1 Sequence, position and specificity of primers used in this study

Assay	Primer	Sequence 5’to 3’	Position^a	Amplicon size
Standard DNA	F1	ATTATTTGTAAAAGTTGCGCC	4276–4296	361 bp
Standard DNA	R1	CTAAATCCTATATCAAATACAAGTACAA	4609–4636	361 bp
HRM assay	F2	TCAGATTTTTGGTGGAAAGGTAA	4358–4380	101 bp
HRM assay	R2	TGGTTATCTACATTAATACTCATTTGTTG	4430–4458	101 bp

^aPrimer positions are referred to the sequence of CPV strain Laika-1993 (GenBank accession number: JN033694)

ISSN: 1746-6148