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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis

Fig. 2

Discrimination between CPV and FPV by HRM analysis. FPV and CPV recombinant plasmid DNA, pEASY-FPV-H and pEASY-CPV-2a, were positive control in the qPCR-HRM analysis, and the mixtures of pEASY-FPV-H and pEASY-CPV-2a with different concentration ratios of 1:9 ~ 9:1 (v/v) were also amplified for detecting co-infection. The melt curve was acquired and analyzed by Applied Biosystems® High Resolution Melt Software v3.1. The results show aligned melt curve analysis of pEASY-FPV-H, pEASY-CPV-2a and the mixtures of the two standard plasmids at the ratio of 1:1 (v/v)

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BMC Veterinary Research

ISSN: 1746-6148

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