Longitudinal study of humoral immunity to bovine coronavirus, virus shedding, and treatment for bovine respiratory disease in pre-weaned beef calves

Table 4 Sequence analysis of the BCV spike gene. Sequence analysis of a 1102 nucleotide fragment of the BCV spike gene spanning the S1 hypervariable region and antigenic domain II

	Position in Mebus spike peptide (U00735.2)^a
	458	465	470	476	484	492	509	510	525	531	535	543	546	571	578	628
Mebus	F	V	H	A	S	D	N	S	H	N	T	S	P	Y	T	L
1701_PC_1^b	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2018_PC_1	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2507_PC_2	S	A	D	V	T	G	T	T	Y	D	T	A	S	H	S	L
2369_PC_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2785_PC_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2830_PC_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
1399_PC_3	F ^c	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	F
4150_PC_3	S	A	D	A	T	G	T	T	Y	D	I	A	S	H	S	L
2402_MT_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2507_MT_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2812_MT_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2815_MT_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2826_MT_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2830_MT_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L
2721_W_2	S	A	D	A	T	G	T	T	Y	D	T	A	S	H	S	L

^aInferred polypeptide sequences were aligned with the reference Mebus genome (Genbank accession U00735.2) corresponding to amino acids 396 to 762, and only those amino acid positions differing from the reference Mebus strain are pictured
^bStrains from study calves are listed as: animal ID_sample acquisition time_herd number. PC = preconditioning, MT = mass treatment, W = weaning
^cBold letters are the four amino acid changes inferred by the single nucleotide polymorphisms identified by Sanger sequencing

ISSN: 1746-6148