Fig. 3
Expression of NDRG1 in epithelia. Strong signal is present in the epithelium of the colon (a) and jejunum (b) of both the control and NDRG1mut/mut Alaskan malamute, whereas NDRG1 staining in the epithelial cells of the bile ducts in the liver was only detected in the control dog (c). NDRG1 is also present in the endometrium (d) and the epithelium of the prostate (e). In the lungs (f), the club cells (arrows) of the bronchiolar epithelium display a more intense signal than the surrounding epithelial cells. In addition, signal from the endothelium (arrowhead) can be seen. Note that except for the lack of signal in the bile ducts, the NDRG1 staining in the NDRG1mut/mut Alaskan malamute is similar to the controls. The extensive yellow-brown granules in the bile ducts of the NDRG1mut/mut Alaskan malamute is interpreted as pigment deposits. Bar 50 μm