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Table 2 Primers used in the RT-PSR and RT-PCR assay for porcine epidemic diarrhea virus (PEDV) detection

From: Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method

Primer

Sequence

Position within PEDV ORF3a

PSR-1

5′-TATTATGTTGGCAGCGCGTT-3′

207–226

PSR-2

5′-TGCCGTCATAATAAGCTGCT-3′

424–405

PSR-S1

5′-ACGAATTCGTACATAGAAGTATAG-TATTATGTTGGCAGCGCGTT-3′

207–226

PSR-S2

5′-GATATGAAGATACATGCTTAAGCA-TGCCGTCATAATAAGCTGCT-3′

424–405

P1

5′-GTCTGCTTTTACTCCTGGCG-3′

325–344

P2

5′-CTCAACAGTTCGCAACAGCT-3′

564–545

  1. The text shown in italics in PSR-S1 and PSR-S2 is the central sequence, which is the same in PSR-1 and PSR-2, respectively, and the bold text is the EcoR I restriction site
  2. aThe primer position is based on the sequence of the HeN/MY/2015 strain, GenBank accession number: KU641647
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BMC Veterinary Research

ISSN: 1746-6148

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