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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method

Fig. 2

Determination of RT-PSR specificity. Lane M, bases pair (bp) marker DL2000; lane 1, PEDV; lane 2, CSFV; lane 3, PRRSV; lane 4, TGEV; lane 5, PRV; lane 6, PCV2; lane 7, PPV; and lane 8, negative control. a Specificity of the RT-PSR assay as determined by electrophoretic separation of the reaction products. b Colorimetric analysis of the reactions demonstrating the specificity of the RT-PSR assay. c Identification of the RT-PSR products by enzyme digestion. Lane M, molecular size marker DL2000; Lane 1, RT-PSR products; Lane 2: EcoR I-digested RT-PSR products

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