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Table 2 The effect of TC (41 °C for 6 h; Post- Hatch Day 28) during 4 time terminals (at 0, 1, 3 and 5 h of TC) on relative mRNA expression of Hsp108, Hsp90, HSF-1 and HSF-2 in the Muscle of broiler chicks subjected to different TM protocols at ED 12–18 (n = 5)

From: Basal and dynamics mRNA expression of muscular HSP108, HSP90, HSF-1 and HSF-2 in thermally manipulated broilers during embryogenesis

Parameters

Groups

Treatments

 

Control

TM1

TM2

TM3

TM4

Hsp108

TN

1.00 ± 0.04aw

6.55 ± 0.70bw

2.15 ± 0.06cw

2.04 ± 0.14cw

2.89 ± 0.19dw

 

TC 1

11.32 ± 0.50ax

8.11 ± 0.22bx

14.57 ± 0.13cx

11.37 ± 0.04ax

13.18 ± 0.16dx

 

TC 3

15.74 ± 0.02ay

21.56 ± 0.06by

13.18 ± 0.25cy

13.77 ± 0.24cy

31.36 ± 0.62dy

 

TC 5

14.81 ± 0.24az

7.96 ± 0.19bz

12.60 ± 0.05cz

14.39 ± 0.19az

9.15 ± 0.04dz

Hsp90

TN

1.00 ± 0.06aw

4.94 ± 0.36bw

2.35 ± 0.05cw

2.06 ± 0.04cw

2.83 ± 0.12dw

 

TC 1

11.74 ± 0.60ax

7.68 ± 0.10bx

13.99 ± 0.07cx

11.18 ± 0.20ax

12.67 ± 0.20dx

 

TC 3

17.35 ± 0.49ay

20.41 ± 0.59by

13.32 ± 0.29cx

13.52 ± 0.28cy

30.22 ± 0.92dy

 

TC 5

15.47 ± 0.16az

7.54 ± 0.08bx

12.10 ± 0.14cy

13.22 ± 0.08dy

8.97 ± 0.25ez

HSF-1

TN

1.00 ± 0.08 aw

1.16 ± 0.08aw

1.95 ± 0.07bw

1.02 ± 0.05aw

1.40 ± 0.08cw

 

TC 1

2.07 ± 0.04ax

2.52 ± 0.12bx

2.23 ± 0.02cx

1.83 ± 0.01dx

1.94 ± 0.06ax

 

TC 3

3.93 ± 0.11ay

2.24 ± 0.03bx

1.82 ± 0.07cw

1.23 ± 0.01dw

3.09 ± 0.03ey

 

TC 5

1.67 ± 0.04ax

1.68 ± 0.04ay

2.83 ± 0.02by

1.99 ± 0.04cx

3.45 ± 0.05dz

HSF-2

TN

1.00 ± 0.04aw

3.79 ± 0.33bw

1.57 ± 0.04cw

1.40 ± 0.07cw

2.23 ± 0.07dw

 

TC 1

2.26 ± 0.06ax

2.72 ± 0.10bx

3.08 ± 0.02cx

1.43 ± 0.01dw

3.33 ± 0.07ex

 

TC 3

3.14 ± 0.04ay

2.17 ± 0.01by

1.61 ± 0.02cw

2.70 ± 0.02dx

5.72 ± 0.09ey

 

TC 5

3.01 ± 0.07ay

5.27 ± 0.25bz

2.93 ± 0.03ax

3.18 ± 0.15ay

3.99 ± 0.06cz

  1. TC Thermal challenged, TM thermal manipulation, TN Naїve (thermal-neutral). Control = 37.8°C; TM1 = Thermal manipulation at 38.5°C for 18 h; TM2 = Thermal manipulation at 39 °C for 18 h; TM3 = Thermal manipulation at 39.5°C for 18 h; TM4 = Thermal manipulation at 40 °C for 18 h
  2. a–ewithin rows, means ± SD with different superscripts differ significantly (P < 0.05)
  3. w-zBetween naïve and TC chicks within a parameter, means ± SD with different superscripts differ significantly (P < 0.05)