Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12917-019-1827-7/MediaObjects/12917_2019_1827_Fig3_HTML.png)
Effect of different thermal manipulation protocols (TM1: TM2: TM3 and TM4) daily during the embryonic days (ED) 12–18 on the mRNA expressions of a) Hsp108, b) Hsp90, c) HSF-1 and d) HSF-2 on post-hatch day 1. Control = 37.8°C; TM1 = Thermal manipulation at 38.5°C daily for 18 h; TM2 = Thermal manipulation at 39 °C daily for 18 h; TM3 = Thermal manipulation at 39.5°C daily for 18 h and TM4 = Thermal manipulation at 40 °C daily for 18 h. a–d Within the same gene, means ± SD with different superscripts differ significantly (p < 0.05)