Fig. 1From: Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducksResponse surface plots for DHAV. Different response plots for DHAV for various combinations of the investigated parameters. a: Different combinations of four factors to form the three-dimension response surface curves; Mg: Mg2+ concentration, Tm: the annealing temperature, DNA pol: Taq DNA polymerase concentration, dNTP: dNTP concentration. b: 4D plots indicated the interaction between the four factors Back to article page