Fig. 4

a A portion of the DNA sequence chromatogram CYP2R1 exon 5 (shown 3′ → 5′ sequence), which shows the T deletion at nucleotide position c.1386 (arrow) and the heterozygous variant at position c.1380 (arrow). b Protein sequence alignment. The normal protein has 501 amino acids, but the sequence from the affected cat is altered from position 462 on, and a stop codon at position 481 truncates the protein