Fig. 4

Murine xenograft sarcomas from ISS cells have vimentin immunoreactivity and lack cytokeratin immunoreactivity. Immunolabeling for vimentin (1:80, DAKO monoclonal mouse anti-vimentin, M7020) and cytokeratin (1:200, DAKO monoclonal mouse anti-human CKAE1/3 antibody, M3515) was performed by the Cornell University Animal Health Diagnostic Center Histology Laboratory (Ithaca, NY) using Leica Bond Max Automated IHC Staining System. Sarcomas that formed at the site of injection of either B4 (a) or C10 (b) cells showed moderate to strong cytoplasmic immunoreactivity for vimentin. No immunoreactivity was detected for cytokeratin in sarcomas that formed at the site of injection of either B4 (c) or C10 (d) cells. Bar = 60 μm