Table 2 PCR primer used for detection of Colicin genes
Name of Colicin | Primer Sequences (5′ to 3′) | Band Size (bp) | Tm (°C) |
|---|---|---|---|
Colicins A, N, S4 | CGT AGC TAT AAT GAA GCA ATG GCT TCA ACC TCC AAC AGG AGA GGT CCC CAG TT | 225 | 57 |
Colicin V | CAC GCC CTG AAG CAC CAC CA CCG TTT TCC AAG CGG ACC CC | 400 | 68 |
Colicins Ιa, Ιb | GCA CAA CAG GCC CGT CTG CTC CAC CTT CCA CAT CCT CTG TCA CC | 385 | 68 |
Colicins E2, E3, E4, E5,E6, E7, E8, E9 | CGA CAG GCT AAA GCT GTT CAG GT TGC AGC AGC ATC AAA TGC AGC CT | 219 | 60 |
Colicins U, Y | GTG AAC GGA CAG AAA CCC GCC CAA TCT GTC TGA CAG CCT CTC CC | 243 | 68 |
Colicin 5, 10, K | AAA GCT GAA CTG GCG AAG GC CAA CTC ATC ATC CCC TAT GTA AGA AG | 803 | 60 |
Colicin E1 | ACG GGA GTG GCT CTG GCG G CTC TTT ACG TCG TTG TTC TGC TTC CTG | 389 | 68 |