Comparison of the sensitivity of three cell cultures to ORFV

BMC Veterinary Research

Table 2 Bovine Sertoli cell growth properties and virus replication

Cell passage	Cell growth time (to a confluent monolayer, h)	Time to 75% CPE (h)	Virus titers at 75% CPE (log TCID50/0.1 ml)
3	72.16 ± 0.29	66.33 ± 0.29	6.47 ± 0.06
4	72.33 ± 0.29	65.50 ± 0.50	6.32 ± 0.03
5	72.50 ± 0.53	68.16 ± 0.29	6.35 ± 0.05
6	72. 33 ± 0.29	67.33 ± 29	6.42 ± 0.08
7	72.16 ± 0.29	69.50 ± 0.5	6.45 ± 0.05
8	72.16 ± 0.29	65.33 ± 0.29	6.35 ± 0.05
9	72.50 ± 0.53	65.50 ± 0.50	6.42 ± 0.03
10	78.33 ± 0.29	65.33 ± 0.29	6.37 ± 0.06
11	80.50 ± 0.50	70.00 ± 1.00	6.42 ± 0.08
12	85.33 ± 0.29	68.50 ± 0.53	6.40 ± 0.007
13	90.33 ± 0.29	66.33 ± 0.29	6.38 ± 0.104
14	92.50 ± 0.53	65.00 ± 1.00	6.35 ± 0.05
15	96.33 ± 0.29	69.33 ± 0.29	6.35 ± 0.05
16	105.16 ± 0.29	72.00 ± 1	6.00 ± 0.1*
17	105.67 ± 0.36	65.50 ± 0.50	5.9 ± 0.1*
18	110.33 ± 0.29	68.50 ± 0.53	5.80 ± 0.1*
19	120.33 ± 0.29	66.33 ± 0.29	5.52 ± 0.08*
20	120.50 ± 0.53	69.00 ± 1	5.13 ± 0.25*

Data are expressed as mean ± S.D. (n = 3, each passage). Statistically significant differences in different generations of passaged cells are indicated by *(P ≤ 0.001). The Mann-Whitney U test was used to compare the results of each passage

ISSN: 1746-6148