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Table 2 Bovine Sertoli cell growth properties and virus replication

From: Comparison of the sensitivity of three cell cultures to ORFV

Cell passage Cell growth time (to a confluent monolayer, h) Time to 75% CPE (h) Virus titers at 75% CPE (log TCID50/0.1 ml)
3 72.16 ± 0.29 66.33 ± 0.29 6.47 ± 0.06
4 72.33 ± 0.29 65.50 ± 0.50 6.32 ± 0.03
5 72.50 ± 0.53 68.16 ± 0.29 6.35 ± 0.05
6 72. 33 ± 0.29 67.33 ± 29 6.42 ± 0.08
7 72.16 ± 0.29 69.50 ± 0.5 6.45 ± 0.05
8 72.16 ± 0.29 65.33 ± 0.29 6.35 ± 0.05
9 72.50 ± 0.53 65.50 ± 0.50 6.42 ± 0.03
10 78.33 ± 0.29 65.33 ± 0.29 6.37 ± 0.06
11 80.50 ± 0.50 70.00 ± 1.00 6.42 ± 0.08
12 85.33 ± 0.29 68.50 ± 0.53 6.40 ± 0.007
13 90.33 ± 0.29 66.33 ± 0.29 6.38 ± 0.104
14 92.50 ± 0.53 65.00 ± 1.00 6.35 ± 0.05
15 96.33 ± 0.29 69.33 ± 0.29 6.35 ± 0.05
16 105.16 ± 0.29 72.00 ± 1 6.00 ± 0.1*
17 105.67 ± 0.36 65.50 ± 0.50 5.9 ± 0.1*
18 110.33 ± 0.29 68.50 ± 0.53 5.80 ± 0.1*
19 120.33 ± 0.29 66.33 ± 0.29 5.52 ± 0.08*
20 120.50 ± 0.53 69.00 ± 1 5.13 ± 0.25*
  1. Data are expressed as mean ± S.D. (n = 3, each passage). Statistically significant differences in different generations of passaged cells are indicated by *(P ≤ 0.001). The Mann-Whitney U test was used to compare the results of each passage