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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Purification of recombinant IpaJ to develop an indirect ELISA-based method for detecting Salmonella enterica serovar Pullorum infections in chickens

Fig. 1

Expression and purification of recombinant MBP-IpaJ. (a) Amplified ipaJ product for construction of the prokaryotic recombinant plasmid pMAL-c5X-ipaJ. Lane 1: plasmid pMAL-c5X-ipaJ digested with NdeI and EcoRI (Takara, Japan). (b) Expression of recombinant protein MBP-IpaJ. Lane M: the size of protein ladders (kDa) shown in the figure are 130, 95, 72 (red), 55, 43, 34, 26, and 17, respectively. Lane 1: induced ER2523-pMAL-c5X as negative control; Lane 2: the supernatant of non-induced ER2523-pMAL-c5X-ipaJ; Lane 3: the supernatants of ER2523-pMAL-c5X-ipaJ induced at 30 °C for 4 h; Lane 4: the supernatants of ER2523-pMAL-c5X-ipaJ induced at 37 °C for 4 h. (c) purified recombinant MBP-IpaJ

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BMC Veterinary Research

ISSN: 1746-6148

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