Fig. 4

Localization of Grp78/BiP to suprabasal keratinocytes of the epidermal lamellae in laminitic tissue. Tissue sections were stained as described in Methods to localize Grp78/BiP (green) and wheat germ agglutinin (WGA; red) to outline cell plasma membranes (epidermis) and extracellular matrix (dermis). The Grp78/BiP channel is also shown separately in grayscale for each image. Representative images from Abaxial (AbAx), Middle (Mid) and Axial positions (Ax) are shown for control and laminitic samples. The control tissues showed no significant Grp78/BiP staining under the conditions used. In contrast, laminitic tissues showed bright Grp78/BiP expression in suprabasal epidermal keratinocytes located at the abaxial region and along the keratinized axis. Positive staining was absent from SELs in the axial region. All images are shown at the same magnification. Scale bar is 50 μm