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Table 1 List of primer sequences used in the study

From: Molecular characterization and expression analysis of two peptidoglycan recognition proteins (CcPGRP5, CcPGRP6) in larvae ontogeny of common carp Cyprinus carpio L. and upon immune stimulation by bacteria

Name

Sequence

Application

PGRP5-F

5′-CCTCAGCTCGCTCACATCCA-3′

cDNA amplification

PGRP5-R

5′-AGAGTATAAATGTTCCCCCGGA-3′

PGRP6-F

5′-GTGTACACTCTCTCTGGTTTGC-3′

PGRP6-R

5′-CTGGTAACGCTCCCATGTCTGG-3′

β-actin-F

5′-TGGCATCACACCTTCTACAAC-3′

β-actin-R

5′-GCCCATCTCCTTGCTCGAAGTC-3′

5′RACE Outer primer

5′-CATGGCTACATGCTGACAGCCTA-3′

RACE gene specific primers

5′RACE Inner primer

5′-CGCGGATCCACAGCCTACTGATGATCAGTCGATG-3′

3′RACE Outer primer

5′-TACCGTCGTTCCACTAGTGATTT-3′

3′RACE Inner primer

5′-CGCGGATCCTCCACTAGTGATTTCACTATAGG-3′

5′ PGRP5 Outer primer

5′-TGGATGTGAGCGAGCTGAGGGACG-3′

5′ PGRP5 Inner primer

5′-ATCACAGTCTGTGCGGGACTCTTC-3′

3′ PGRP5 Outer primer

5′-CGTCCCTCAGCTCGCTCACATCCA-3′

3′ PGRP5 Inner primer

5′-GGAGATGGGATGGTGTATGAAGGG-3′

5′ PGRP6 Outer primer

5′-GTAACTCTTCAGCAGGTCACTCAG-3′

5′ PGRP6 Inner primer

5′-AACTTCTGACCCGAGAATGAACCC-3′

3′ PGRP6 Outer primer

5′-CAAGAGTGCGATGGACATGGTGAG-3′

3′ PGRP6 Inner primer

5′-GTCTTCATCATACTCCTTATACGG-3′

PGRP5-qF

5′-CCTCAGCTCGCTCACATCCA-3′

Primers for Real-time PCR

PGRP5-qR

5′-CGCCCTTCATACACCATCCC-3′

PGRP6-qF

5′-ATGGTGAGGTATGACTTC-3′

PGRP6-qR

5′-CTTGTCTGTGTCCGTATA-3′

S11-F

5′-CCGTGGGTGACATCGTTACA-3′

S11-R

5′-TCAGGACATTGAACCTCACTGTCT-3′

  1. All the primer sequences used in gene clone and Real-time quantitative PCR of Ccpgrp5, Ccpgrp6 and internal reference gene