Fig. 5

Diagram explaining the experimental design used in our study. F-C: MII denuded oocytes were cultured in KSOM medium for 2 h prior to IVF; V-C: MII denuded oocytes were cultured in KSOM medium for 2 h prior to vitrification. After warming, oocytes were allowed to recover in KSOM medium for 2 h and subjected to IVF; V-NAC-Pre: MII denuded oocytes were cultured in KSOM medium supplemented with 1 mM NAC for two hours prior to vitrification. Warmed oocytes were allowed to recover for 2 h in KSOM medium before IVF; V-NAC-Post: MII denuded oocytes were cultured in KSOM medium for 2 h prior to vitrification. Warmed oocytes were allowed to recover for 2 h in KSOM medium added with 1 mM NAC before IVF