Fig. 2

Identification of TBDT in Avibacterium paragallinarum cultures using the 1G7G8 monoclonal antibody in Western blot. Avibacterium paragallinarum cultures: FARPER-113 (serogroup B), FARPER-114 (serogroup A) and FARPER-140 (serogroup C). Lane M: Molecular weight marker (26.6–180 kDa). For bacterial lysate concentrations: lane 1 (500 μg/mL), lane 2 (50 μg/mL), lane 3 (5 μg/mL), lane 4 (0.5 μg mL), lane 5 (0.05 μg/mL), and lane 6 (0.005 μg/mL). For bacterial pellet: lane 1 (500 μg/mL), lane 2 (50 μg/mL), lane 3 (5 μg/mL), lane 4 (0.5 μg mL), lane 5 (0.05 μg/mL), and lane C (recombinant TBDT). For bacterial supernatant concentrations: (a) (undiluted supernatant), (b) (diluted 1:2), (c) (diluted 1:4), (d) (diluted 1:8), (e) (diluted 1:16), and (f) (BHI broth). Black arrows indicate the specific reactive band in each lane