Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis

Table 1 Primer and probe sequences used for RPA-LFD assay

Name	Sequence (5′-3′)	Genome location	Amplification size (bp)
uvrC-F1	TAAATGAGCGCAGTGCTGATGTTGAAT	1464–1490	281
uvrC-F2	GTAGCAACAAAAACACTAAAGATTATGACT	1521–1550	224
uvrC-F3	CTTAAAACGCTTAATATAAACATCCCTGT	1601–1629	144
uvrC-F4	AACGCTTAATATAAACATCCCTGTTATTG	1606–1634	139
uvrC-R	Biotin-AACTTGAATTTGAACTAAGTAGTTGTATAG	1715–1744
uvrC-LF Probe	FAM-AAATGAGTTTCACAAAACCAAAGCCTTAAT [dSpacer]GACCTAGATATGAATGA-C3 Spacers	1645–1692	100
oppD-oppF-F1	TTGAACAAATACGTCAAGAGTACAATATA	22–50	234
oppD-oppF-F2	CAATATATCAATAATTTTAATTTCGCATAACA	44–75	212
oppD-oppF-F3	TGTTATGTATGCTGGCAAAATTGTTGAAAGA	110–140	146
oppD-oppF-R	Biotin-CTGGTGGGGTTCCTTGAATTGAGAATAATCT	225–255
oppD-oppF-LF Probe	FAM-TCCAGCTCACCCTTATACATGAGCGCTTATC [dSpacer]CGGCTATACCTGAA-C3 Spacers	167–212	89

Abbreviations: FAM is 6-Carboxyfluorescein, dSpacer is an exonuclease site, and C3 Spacers is a polymerase extension blocking site

ISSN: 1746-6148