Table 7 Primers for RT-qPCR genes based on the ovis sequences obtained from the NCBI database
Gene |  | Primer sequences (5′–3′) | Amplicon size | NCBI accession no. |
|---|---|---|---|---|
CXCL8 | F: | AGAGAGCTGAGAAGCAAGATCCA | 150 bp | NM_001009401.2 |
| Â | R: | CCCTACACCAGACCCACACA | Â | Â |
IL-1β | F: | GGCAGAAGGGAAGGGAAGA | 81 bp | NM_001009465 |
| Â | R: | AATACAGGGGAGGCAGTTGG | Â | Â |
GRα | F: | TGCCAAGGGTCTGGAGATG | 132 bp | NM_001114186.1 |
| Â | R: | TGAGGAACTGGATGGAGGAGA | Â | Â |
TLR4 | F: | GACCCTTGCGTACAGGTTGTT | 80 bp | NM_001135930.1 |
| Â | R: | GGGATGTTGTCGGGGATTT | Â | Â |
TNFα | F: | ACGGCGTGGAGCTGAAA | 132 bp | NM_001114186.1 |
| Â | R: | CTGATGGTGTGGGTGAGGAA | Â | Â |
IFN-γ | F: | TGGAGGACTTCAAAAGGCTGA | 183 bp | NM_001009803.1 |
| Â | R: | GCAGGCAGGAGAACCATTACA | Â | Â |
Fas | F: | GATATTGCTTGGCTTGGCTTT | 167 bp | NM_001123003.1 |
| Â | R: | CCAGCATTCATCTCCCCAAC | Â | Â |
NFκB1 | F: | AGCACCACTTATGACGGAACTACA | 168 bp | XM_004009667.3 |
| Â | R: | GACCCCTTCATCCTCTCCATC | Â | Â |
NFκB2 | F: | GGAGGCCAAGGAACTGAAGA | 101 bp | XM_004020143.3 |
| Â | R: | TCAGGGGCAGAGAGAAGGAG | Â | Â |
CD62L | F: | CGGAGAAGCACGGTTGATG | 198 bp | XM_012187246.2 |
| Â | R: | CAAAGAGGGGACAGAAGGAGAAG | Â | Â |
β-actin | F: | CCTGCGGCATTCACGAA | 134 bp | NM_001009784.2 |
| Â | R: | GCGGATGTCGACGTCACA | Â | Â |