Agrimonia procera exerts antimicrobial effects, modulates the expression of defensins and cytokines in colonocytes and increases the immune response in lipopolysaccharide-challenged piglets

Table 3 Characteristics of primer sequences used for quantitative real-time RT-PCR analysis

Gene	Forward primer (from 5′ to 3′) Reverse primer (from 5′ to 3′)	Product size (bp)	NCBI GenBank number
CXCL1	ATGCTGAACAGTGACAAATC	96	NM_001511.3
CXCL1	TCTTCTGTTCCTATAAGGGC	96	NM_001511.3
IL-8	GTTTTTGAAGAGGGCTGAG	89	NM_000584.4
IL-8	TTTGCTTGAAGTTTCACTGG	89	NM_000584.4
GPX2	AATTTGGACATCAGAACTGC	190	NM_001115136.1
GPX2	GGCTGCTCTTCAAGATTTAG	190	NM_001115136.1
DEFB1	AGGTGGTAACTTTCTCACAG	192	NM_005218.3
DEFB1	AAGTTCATTTCACTTCTGCG	192	NM_005218.3
GAPDH	GACCACAGTCCATGCCATCAC	453	NM_002046.5
GAPDH	TCCACCACCCTGTTGCTGTAG	453	NM_002046.5
RPLP0	TCGACAATGGCAGCATCTAC	223	NM_001002.3
RPLP0	GCCTTGACCTTTTCAGCAAG	223

CXCL1 Chemokine (C-X-C motif) ligand 1, IL-8 Interleukin-8, GPX2 Glutathione peroxidase 2, DEFB1 Beta-defensin 1, GAPDH Glyceraldehyde 3-phosphate dehydrogenase, RPLP0 Ribosomal phosphoprotein P0

ISSN: 1746-6148