Table 2 Primer sequences and reference genes used for PCR
From: Transcripts of antibacterial peptides in chicken erythrocytes infected with Marek’s disease virus
Target Genes | Primer sequence(5′ → 3′) | Annealing temperature | Sizes(bp) | GenBank Accession number of the reference gene |
|---|---|---|---|---|
AvBD1 | F: GGCTCTAGGAAGGAAGTCA R: GCAGCAGAGGTAAAATCTTG | 57 °C | 112 | NM_204993.1 |
AvBD2 | F: GCACTCCAGGTTTCTCCA R: GGCGTCCGACTTTGATTA | 57 °C | 110 | DQ677633.1 |
AvBD3 | F: AGAGGAGGATTCTGTCGTGTT R: CAACCTCATATGCTCTTCCA | 55 °C | 100 | NM_204650.2 |
AvBD4 | F: TCATGGAGCTGTGGGCTTTT R: AGCATTCCCATAAGGGCATT | 55 °C | 100 | NM_001001610.2 |
AvBD5 | F: ATTACCCCAGGACTGTGA R: GCAGCAGAAGTCTTCCTT | 55 °C | 100 | NM_001001608.2 |
AvBD6 | F: CTGCTGCTGTCTGTCCTCTT R: TGCAGACACCCCTTTGATAT | 55 °C | 100 | NM_001001193.1 |
AvBD7 | F: CTATTGATACTTGTTGGCTTCG R: AACTCCTCCATCCCCTTG | 57 °C | 122 | AY621322.1 |
AvBD9 | F: CCAGCTTACAGCCAAGAAGA R: TCCCAATGTCAACTGAAGGA | 55 °C | 100 | NM_001001611.2 |
LEAP-2 | F: ACTCTGGAATTCTGCCTGATGACA R: CATCTGCATCCGTGCCTGA | 57 °C | 66 | NM_001001606.1 |
18S rRNA | F: TTCCGATAACGAACGACAC R: GACATCTAAGGGCATCACAG | 55 °C | 139 | FM165414 |