Profiling of rumen fermentation, microbial population and digestibility in goats fed with dietary oils containing different fatty acids

Table 5 The PCR primer used for quantification of rumen microorganism

Microbes	Primer		Amplicon (base pairs)	Ref
Microbes	Forward	Reverse	Amplicon (base pairs)	Ref
General bacteria	5’-CGGCAACGAGCGCAACCC-3′	5’-CCATTGTAGCACGTGTGTAGCC-3′	130	[56, 57]
Fibrobacter succinogenes	5’-GTTCGGAATTACTGGGCGTAAA-3′	5’-CGCCTGCCCCTGAACTATC-3′	121	[56, 57]
Ruminococcus albus	5’-CCC TAA AAG CAG TCT TAG TTC G-3’	5’-CCT CCT TGC GGT TAG AAC A-3’	175	[58]
Ruminococcus flavefaciens	5’-CGAACGGAGATAATTTGAGTTTACTTAGG-3′	5’-CGGTCTCTGTATGTTATGAGGTATTACC-3′	132	[56, 57]
Methanogenic archea	5’-TTCGGTGGATCDCARAGRGC-3′	5’-GBARGTCGWAWCCGTAGAATCC-3′	140	[19]
Protozoa	5’-GCTTTCGWTGGTAGTGTATT-3′	5’-CTTGCCCTCYAATCGTWCT-3′	223	[19]

ISSN: 1746-6148