Table 1 Porcine specific primers used for gene expression analysis
Genes | Primer sequences | Products |
|---|---|---|
size (bp) | ||
CreER T2 | 5’-TCGCAAGAACCTGATGGACA-3′ | 288 |
5’-CGCCGCATAACCAGTGAAAC-3′ | ||
eGFP | 5’-AACGGCCACAAGTTCAGCGT-3′ | 424 |
5′- TCACCTTGATGCCGTTCTTC -3’ | ||
Notch1 | 5’-TGGATGGCATCAATTCCTTT-3’ | 138 |
5′- ACTTGTAGGCGCCATAGCTG-3’ | ||
Hes5 | 5’-TTCTCAGAGAATGTGTGTGCAGAGT-3’ | 75 |
5’-GGTCAGACACTTGGCAGAAGATG-3’ | ||
Sox2 | 5’-CGGCGGCAGGATCGGC-3’ | 113 |
5’-GAGCTCCGCGAGGAAAA-3’ | ||
GFAP | 5’-CACAAGGATGTGGTGTGAGG-3’ | 207 |
5’-TGCTACCTGGCAGGCTTAAT-3’ | ||
Ki67 | 5’-GAAACCCAGATCCGAGCATA-3’ | 139 |
5’-CAGCAGCTATTCTGGCAACA-3’ | ||
GAPDH | 5’-GTCGGTTGTGGATCTGACCT-3’ | 207 |
5’-TTGACGAAGTGGTCGTTGAG-3’ | ||
RN18S | 5’-CGCGGTTCTATTTTGTTGGT-3’ | 219 |
5’-AGTCGGCATCGTTTATGGTC-3’ |