Table 2 Primers used in this study
Name | Targeted region | Sequence (5′ → 3′) | Size (bp) |
|---|---|---|---|
Col3a1-B1-SacI-F Col3a1-B1-Hind III -R | 3’ UTR of Col3a1–1 | cgagctcAACAAAATTTCTGGGGGAAAA cccaagcttCTGGGAGCAATCAGTGCTTT | 225 |
Col3a1-B2-SacI-F Col3a1-B2-Hind III -R | 3’ UTR of Col3a1–2 | cgagctcTCCTGTCATTGCTGGTCAAG cccaagcttCATGCCAATGACAATCTTTGA | 180 |
Insig1-SacI-F Insig1-HindIII -R | 3’ UTR of Insig1 | cgagctcTGCCCAAAGGAAGTAGATCG cccaagcttACCCCCTCCACTGCTAAAGT | 222 |
Sgk1-SpeI-F Sgk1-MluI-R | 3’ UTR of Sgk1 | cgactagtTTTGTTCTTCCCCCAAAGTG cgacgcgtCACACTCTGCAACAGGGAAA | 227 |
Sgk1-F Sgk1-R | Sgk1 CDS | CCCCCTTTTAACCCAAATGT GGCATATGAGAAGCCCAAAA | 165 |
Col3a1-F Col3a1-R | Col3a1 CDS | AGGCTGAAGGAAACAGCAAA GTCCACGCCAAATTCTTGAT | 176 |
Insig1-F Insig1-R | Insig1 CDS | CTGGGTCTCTGGTGGACATT GGAAGCCAAGAACGGATGTA | 155 |
Ppm1d -F Ppm1d -R | PPM1D CDS | GGCATATGAGAAGCCCAAAA CCACGACTGAATGCTTCTGA | 227 |
Ddx3x -F Ddx3x -R | DDX3X CDS | GAAAGTTTGCATACCGTTCCA CAGTCCAATCTTTCCCCTCTC | 155 |
Tnfaip3-F Tnfaip3-R | TNFAIP3 CDS | GCAGATTCGAGGATTTGAGC TTGGGTAGGTTGCCTTCATC | 169 |
Gapdh-F Gapdh-R | GAPDH CDS | GCCATCAATGATCCCTTCAT CTGGGGTCACGCTCCTG | 155 |
F-miR-29c-F F-miR-29c-R | miR29c upstream sequence | ATTACAGCGTGGGAGGAGAC AGAGACGTTTTCCTGGCTCA | 863 |