Fig. 8
Effect of TGFβ receptor I inhibition by LY2157299 on TAZ levels and cell proliferation in D17 cells. Representative immuoblots and densitometry analysis of TAZ and pSmad3 protein levels when TAZ levels were depleted (siTAZ) or not (siCtrl), and cells were treated with nothing (Control), 5 ng/mL TGFβ (+ TGFβ), 10 μM of LY2152799 (+ LY) or combination treatment (+ TGFβ + LY). Experimental groups were normalized to loading control β-actin. Graphs depict the average fold change in TAZ or pSmad3 expression relative to siCtrl non-treated group ± SEM from two independent experiments (a). LY inhibited Smad3 phosphorylation and decreased TAZ levels compared to non-LY treated cells, suggesting that suppressing active TGFβ signaling could decrease TAZ levels in D17 cells. b Representative immunofluorescence images of the mitotic marker phospho-histone H3 (pHH3) taken at 20X objective lens and graph depicting the relative percentage of pHH3 positive cells; bars depict average ± SEM from three independent experiments. Scale bar = 100 μm