Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle

Table 1 PCR and DNA sequencing primers

Primer	Sequence (5′ → 3′)	PCR conditions^a			Size (bp)	Reference
Primer	Sequence (5′ → 3′)	D (s)	A (°C, s)	E (s)	Size (bp)	Reference
rrs-F	CGT GGC CGT TTG TTT TGT CA	30	55, 30	90	1744	This study
rrs-R	AAG TCC GAG TGT TGC CTC AG
rrs-seq1	GAA GAA GCA CCG GCC AAC TA
rrs-seq2	TTG TAC CGG CCA TTG TAG CA
rpsL-F	ATG AGA CGA ATC GAG TTT GAG	30	55, 30	60	632	[44]
rpsL-R	GCT CAA GCG CAC CAT AAA CAA	30	55, 30	60	632	[44]
gidB-F	CGC CGA GTC GTT GTG CT	30	55, 30	60	892	[44]
gidB-R	AGC CTG GCC CGA CCT TA	30	55, 30	60	892	[44]

^aEach PCR assay includes an initial denaturation step at 95 °C for 15 min to fully denature the template, followed by 35 cycles of denaturation, annealing and extension steps. D, denaturation time in s at 95 °C; A, annealing conditions (temperature and time in s); extension time in s

ISSN: 1746-6148