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Table 1 PCR and DNA sequencing primers

From: Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle

Primer Sequence (5′ → 3′) PCR conditionsa Size (bp) Reference
D (s) A (°C, s) E (s)
rrs-F CGT GGC CGT TTG TTT TGT CA 30 55, 30 90 1744 This study
rrs-R AAG TCC GAG TGT TGC CTC AG
rrs-seq1 GAA GAA GCA CCG GCC AAC TA
rrs-seq2 TTG TAC CGG CCA TTG TAG CA
rpsL-F ATG AGA CGA ATC GAG TTT GAG 30 55, 30 60 632 [44]
rpsL-R GCT CAA GCG CAC CAT AAA CAA
gidB-F CGC CGA GTC GTT GTG CT 30 55, 30 60 892 [44]
gidB-R AGC CTG GCC CGA CCT TA
  1. aEach PCR assay includes an initial denaturation step at 95 °C for 15 min to fully denature the template, followed by 35 cycles of denaturation, annealing and extension steps. D, denaturation time in s at 95 °C; A, annealing conditions (temperature and time in s); extension time in s