Primer | Sequence (5′ → 3′) | PCR conditionsa | Size (bp) | Reference | ||
---|---|---|---|---|---|---|
D (s) | A (°C, s) | E (s) | ||||
rrs-F | CGT GGC CGT TTG TTT TGT CA | 30 | 55, 30 | 90 | 1744 | This study |
rrs-R | AAG TCC GAG TGT TGC CTC AG | |||||
rrs-seq1 | GAA GAA GCA CCG GCC AAC TA | |||||
rrs-seq2 | TTG TAC CGG CCA TTG TAG CA | |||||
rpsL-F | ATG AGA CGA ATC GAG TTT GAG | 30 | 55, 30 | 60 | 632 | [44] |
rpsL-R | GCT CAA GCG CAC CAT AAA CAA | |||||
gidB-F | CGC CGA GTC GTT GTG CT | 30 | 55, 30 | 60 | 892 | [44] |
gidB-R | AGC CTG GCC CGA CCT TA |